Study-unit LABORATORY OF BIOLOGY 3 (INTERDISCIPLINARY)
| Course name | Biological sciences |
|---|---|
| Study-unit Code | GP003063 |
| Curriculum | Cellulare-molecolare |
| Lecturer | Patrizia Rosignoli |
| Lecturers |
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| Hours |
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| CFU | 2 |
| Course Regulation | Coorte 2021 |
| Supplied | 2023/24 |
| Supplied other course regulation | |
| Learning activities | Altro |
| Area | Altre conoscenze utili per l'inserimento nel mondo del lavoro |
| Sector | MED/42 |
| Type of study-unit | Obbligatorio (Required) |
| Type of learning activities | Attività formativa monodisciplinare |
| Language of instruction | Italian |
| Contents | Preparation of cultured cells for electrophysiological studies. Recordings of ionic currents through membrane channels; extraction, quantification of photosynthetic pigments and visible absorption spectra; comparison between sun and shade leaves; sampling and analysis procedures for the examination of some chemical and microbiological parameters in drinking and mineral waters in relation with the potability requirements; Use of high performance liquid chromatography (HPLC) in food quality control. Determination of the main chromatographic parameters; quali-quantitative analysis of bioactive extracts from food matrices. |
| Reference texts | Slides in the UNISTUDIUM web site. |
| Educational objectives | At the end of the course students will have: -gained hands-on experience in a range of practical skills and methodologies relevant to biological research; -appreciated and be practised in the numerical skills necessary to process, analyse and interpret experimental data -understood the principles of experimental design and safe use of materials and equipment in a laboratory context |
| Teaching methods | Practical activity in the laboratory includes a brief introductory lecture which will explain the experimental protocol that all students must follow. Students will carry out experiments individually or in small groups and, at the end, the experimental data obtained will be subject of comparative analysis and discussion. |
| Learning verification modality | Sono previste 5 esercitazioni di laboratorio negli argomenti di seguito riportati per un totale di 24 h. Per il conseguimento dei crediti previsti per questo insegnamento (2 CFU) è obbligatoria la frequenza ad almeno 80% del monte ore complessivo del monte ore complessivo (4 su 5 esercitazioni). La frequenza degli studenti verrà accertata mediante appello e acquisizione delle firme. Il superamento prevede una idoneità. Per informazioni sui servizi di supporto agli studenti con disabilità e/o DSA visita la pagina http://www.unipg.it/disabilita-e-dsa |
| Extended program | The course includes laboratory experiences in which common methods used in biological experiments are discussed, by referring in particular to the content of the teachings of the third year. Experiences will be carried out regarding: -Preparation of cultured cells for electrophysiological studies. Recordings of ionic currents through membrane channels. Data analysis and inferences on the kinetics of ion channels. -Extraction, quantification of photosynthetic pigments and visible absorption spectra for chlorophyll a and chlorophyll b. Rates of electron transport in the thylakoid membranes. - Recombinant DNA Technology: purification and quantification of Nucleic Acids Cloning and recovery of DNA. - Sampling and analysis procedures for the examination of some chemical and microbiological parameters in drinking and mineral waters in relation with the potability requirements. -Use of high performance liquid chromatography (HPLC) in food quality control. Determination of the main chromatographic parameters; quali-quantitative analysis of bioactive extracts from food matrices. |