Study-unit BIOTECHNOLOGY LABORATORY
| Course name | Biotechnology |
|---|---|
| Study-unit Code | A000403 |
| Curriculum | Comune a tutti i curricula |
| CFU | 12 |
| Course Regulation | Coorte 2022 |
| Supplied | 2022/23 |
| Supplied other course regulation | |
| Type of study-unit | Obbligatorio (Required) |
| Type of learning activities | Attività formativa integrata |
| Partition |
BASIC BIOTECHNOLOGY AND ANIMAL MODELS
| Code | A000404 |
|---|---|
| CFU | 6 |
| Learning activities | Caratterizzante |
| Area | Discipline biotecnologiche con finalità specifiche: biologiche e industriali |
| Sector | BIO/05 |
| Type of study-unit | Obbligatorio (Required) |
Canale A
| CFU | 6 |
|---|---|
| Lecturer | Manuela Rebora |
| Lecturers |
|
| Hours |
|
| Language of instruction | Italian |
| Contents | The organizational levels and diversification of the models (acoelomates, pseudocoelomates, coelomates). System functionality and complexity. Reproductive strategies. Organism/environment interactions. Particular attention to the species used as animal models, to the organisms relevant for the biotechnology field, to biomimetics and synthesis of bioactive compounds. |
| Reference texts | Hickman, Roberts, Larson, L'Anson Fondamenti di Zoologia, Diversità Animale Ed. McGraw, Hill |
| Educational objectives | The student will acquire the basic notions of animal biology. The main knowledge gained will be: - Main animal vital functions - Body plan of the main animal Phyla The main skills (the ability to apply the knowledge) will be: - To understaqnd the evolution of the main animal vital functions - To be able to assign an animal model to the right Phylum on the basis of its body plan -To learn how to use the light microscope and the stereomicroscope for the observation of specimens on slide and samples in toto of invertebrates - To learn how to use a dichotomous key for the determination of a zoological preparation -To learn the techniques of breeding, maintenance and manipulation of an insect model, Drosophila melanogaster -To learn the dissection techniques under the stereomicroscope - To provide theoretical and practical skills related to deparaffining and subsequent staining of histological sections on the slide for subsequent assembly and observation under an optical microscope -To provide theoretical and practical skills related to the correct use of the optical microscope for the observation of colored histological slides and the related tissue recognition |
| Prerequisites | Basic knowledge of cellular biology |
| Teaching methods | Theoretical lessons and practical training |
| Other information | Frequency is optional but strongly adviced |
| Learning verification modality | oral exam (two-three questions, half an hour) at the end of the course to verify the acquisition of learning outcomes |
| Extended program | Organizational models: phylum and bauplan, levels of organization, diblastica and triblastica organisation, radial and bilateral symmetry, cephalization, the coelom, the functions of the body cavity, animals acelomati, pseudocoelomates, eucelomati differences protostomes and deuterostomes, the metamerism.The vital functions: integumentary system in invertebrates and vertebrates, colors, skeletal systems, hydrostatic skeleton, rigid skeleton, animal movement, amoeboid movement, ciliary and flagellar movement, pedal locomotion, locomotion with with ambulacral pedicels, with arts, nutrition, types of nutrition, intracellular and extracellular digestion, evolution of the digestive system, circulation, circulatory system, closed and open, evolution of the circulatory system in invertebrates and in vertebrates, respiration, tracheal system of insects, osmoregulation, excretion in aquatic and subaerial animals, evolution of 'excretory system (protonefridi and metanefridi), nervous system, major trends, asexual and sexual reproduction, gametes, sexual dimorphism, determinism of sex, hermaphroditism, parthenogenesis, types of fertilization, oviparous species, ovoviviparous, viviparous. Porifera: organizational models, metabolism, choanocytes, spicules, reproduction, gemmules, in commercial use, role and use of intermediate metabolites. Cnidaria: morphological diversification, polyp and medusa, nematocysts, metabolism, reproduction, class Anthozoa (Octocorals, Hexacorallia, corals), class Scifozoa (life cycles), cubozoans class, class Hydrozoa, the Green Fluorescein Protein (GFP) extracted from jellyfish. Platyhelminthes: metabolism, reproduction, free forms (class Turbellaria) and parasitic forms, class trematodes , class Tapeworms. Aschelminti: pseudocoelom, Eutelia, cryptobiosis, the Nematodes, features, free forms and parasitic forms , Caenorhabditis elegans, the plant parasitic nematodes, nematodes and the biological control. Annelids: the conquest of a true coelom, Polychaeta, Oligochaeta, Hirudinea, general characteristics, the ecological role of the earthworm reproduction in the earthworm. Mollusks: general characteristics, the structure of the shell, class Gastropoda, Bivalvia class, class Cephalopoda. Arthropods: General, Class Arachnids, Crustaceans class, class Diplopoda, Chilopoda class, class Insects, the great evolutionary success of insects, insects and chemical communication, insects and bioluminescence, the societies of insects, and the insects' man, pollinators, pests, biological control, genetic manipulation, the male sterile technique (SIT) and genetically modified organisms, plants BT, insects commercially exploited by man. Echinoderms: general characteristics, class Asteroidea, Ophiuroidea class, class Echinoidea Class Holothuroidea, Crinoidea class. Chordata: subphylum Urochordata, subphylum Cephalochordata, subphylum vertebrates: fish, amphibians, reptiles, birds, mammals. Practical lessons: dissection of an insect. Observation of specimens belonging to the different Animal Phyla, the optical microscope and the stereomicroscope will be used for the observation of specimens on slide and samples of invertebrates, a dichotomous key will be used for the determination of a zoological preparation, the techniques of breeding, maintenance and manipulation of a model insect (Drosophila melanogaster) will be learned, , the techniques of dissection of an invertebrate under the stereomicroscope will be learned, theoretical and practical skills related to the deparaffination and subsequent staining of histological sections on the slide for observation under an optical microscope will be provided, theoretical and practical skills related to the correct use of the optical microscope for the observation of colored histological slides and the related tissue recognition will be provided |
Canale B
| CFU | 6 |
|---|---|
| Lecturer | Silvana Piersanti |
| Lecturers |
|
| Hours |
|
| Language of instruction | Italian |
| Contents | The organizational levels and diversification of the models (acoelomates, pseudocoelomates, coelomates). System functionality and complexity. Reproductive strategies. Organism/environment interactions. Use of the light microscope. Use of histological, histochemical and immuno-cytochemical techniques for preparing and staining histological specimens. Examine and recognize tissue specimens under light microscopy. |
| Reference texts | Hickman C.P., Roberts L.S., Keen S.L., Larson, Larson A, Eisenhour D.J. Fondamenti di Zoologia. Ed. McGraw, Hill Hickman C.P., Roberts L.S., Keen S.L., Larson, Larson A, Eisenhour D.J. Diversità Animale. Ed. McGraw, Hill Slides provided by the teacher. |
| Educational objectives | The student will acquire the basic notions of animal biology. The main knowledge gained will be: - Main animal vital functions - Body plan of the main animal Phyla The main skills (the ability to apply the knowledge) will be: - To understaqnd the evolution of the main animal vital functions - To be able to assign an animal model to the right Phylum on the basis of its body plan - Knowledge and understanding of structural and biologic properties of animal and vegetal cells and tissues - Ability to recognize tissues by examinating histological images of tissues specimens - Ability to use independently the light microscope. The acquired competences will be in the field of biotechnology |
| Prerequisites | In order to be able to understand the arguments treated in the biotechnology lab, students have studied and preferably passed the exam of General Biology. |
| Teaching methods | Theoretical lessons and practical training in laboratory |
| Other information | Frequency is optional but strongly adviced. The schedule of the lessons and the exams are available on the Department's website: http://www.dcbb.unipg.it The teacher can be contacted by e-mail for any need (exam dates, concepts). Additional teaching materials, such as course slides or scientific publications related to the topics discussed, are made available to all students with free access in the uni-studium web site during the course. |
| Learning verification modality | The learning test will be carried out with an oral test lasting about twenty minutes (2-3 questions) on topics covered during the course. The teacher will evaluate the connection of the concepts proposed during the period, asking the student to develop autonomous reasonings demonstrating their complete acquisition and understanding. There are optional ongoing tests during the course, mainly aimed at attending students. For support services for students with disabilities and SLD, visit the link: http://www.unipg.it/disabilita-e-dsa. |
| Extended program | Organizational models: phylum and bauplan, levels of organization, diblastica and triblastica organisation, radial and bilateral symmetry, cephalization, the coelom, the functions of the body cavity, animals acelomati, pseudocoelomates, eucelomati differences protostomes and deuterostomes, the metamerism.The vital functions: integumentary system in invertebrates and vertebrates, colors, skeletal systems, hydrostatic skeleton, rigid skeleton, animal movement, amoeboid movement, ciliary and flagellar movement, pedal locomotion, locomotion with with ambulacral pedicels, with arts, nutrition, types of nutrition, intracellular and extracellular digestion, evolution of the digestive system, circulation, circulatory system, closed and open, evolution of the circulatory system in invertebrates and in vertebrates, respiration, tracheal system of insects, osmoregulation, excretion in aquatic and subaerial animals, evolution of 'excretory system (protonefridi and metanefridi), nervous system, major trends, asexual and sexual reproduction, gametes, sexual dimorphism, determinism of sex, hermaphroditism, parthenogenesis, types of fertilization, oviparous species, ovoviviparous, viviparous. Porifera: organizational models, metabolism, choanocytes, spicules, reproduction, gemmules, in commercial use, role and use of intermediate metabolites. Cnidaria: morphological diversification, polyp and medusa, nematocysts, metabolism, reproduction, class Anthozoa (Octocorals, Hexacorallia, corals), class Scifozoa (life cycles), cubozoans class, class Hydrozoa, the Green Fluorescein Protein (GFP) extracted from jellyfish. Platyhelminthes: metabolism, reproduction, free forms (class Turbellaria) and parasitic forms, class trematodes , class Tapeworms. Aschelminti: pseudocoelom, Eutelia, cryptobiosis, the Nematodes, features, free forms and parasitic forms , Caenorhabditis elegans, the plant parasitic nematodes, nematodes and the biological control. Annelids: the conquest of a true coelom, Polychaeta, Oligochaeta, Hirudinea, general characteristics, the ecological role of the earthworm reproduction in the earthworm. Mollusks: general characteristics, the structure of the shell, class Gastropoda, Bivalvia class, class Cephalopoda. Arthropods: General, Class Arachnids, Crustaceans class, class Diplopoda, Chilopoda class, class Insects, the great evolutionary success of insects, insects and chemical communication, insects and bioluminescence, the societies of insects, and the insects' man, pollinators, pests, biological control, genetic manipulation, the male sterile technique (SIT) and genetically modified organisms, plants BT, insects commercially exploited by man. Echinoderms: general characteristics, class Asteroidea, Ophiuroidea class, class Echinoidea Class Holothuroidea, Crinoidea class. Chordata: subphylum Urochordata, subphylum Cephalochordata, subphylum vertebrates: fish, amphibians, reptiles, birds, mammals. Practical lessons: dissection of an insect. Observation of specimens belonging to the different Animal Phyla, the optical microscope and the stereomicroscope will be used for the observation of specimens on slide and samples of invertebrates, a dichotomous key will be used for the determination of a zoological preparation, the techniques of breeding, maintenance and manipulation of a model insect (Drosophila melanogaster) will be learned, , the techniques of dissection of an invertebrate under the stereomicroscope will be learned, theoretical and practical skills related to the deparaffination and subsequent staining of histological sections on the slide for observation under an optical microscope will be provided, theoretical and practical skills related to the correct use of the optical microscope for the observation of colored histological slides and the related tissue recognition will be provided |
EXPERIMENTAL MODELS OF MICROBIOLOGY AND CELLULAR CROPS
| Code | A000405 |
|---|---|
| CFU | 6 |
| Learning activities | Caratterizzante |
| Area | Discipline biotecnologiche con finalità specifiche:agrarie |
| Sector | AGR/16 |
| Type of study-unit | Obbligatorio (Required) |
Canale A
| CFU | 6 |
|---|---|
| Lecturer | Gianluigi Cardinali |
| Lecturers |
|
| Hours |
|
| Language of instruction | Italian |
| Contents | THEORETICAL PART: Microbiological Techniques PRACTICALS: PART I Laboratory experience PART II Computerized Analysis Tools |
| Reference texts | BROCK. BIOLOGIA DEI MICRORGANISMI. MICROBIOLOGIA GENERALE, AMBIENTALE E INDUSTRIALE. Michael T. Madigan, John M. Martinko |
| Educational objectives | Understanding the structural and functional properties of biological macromolecules, nutrient metabolism and metabolic integrations, regulatory mechanisms operating in biological systems; understanding the structure and biological properties of fungi and bacteria; of the biological structure and properties of cells and animal tissues and methodologies of the molecular analyses. Use and understanding of the animal and microbial models. |
| Prerequisites | Knowledge of mathematical, statistical and bioinformatic methods for quantitative analysis and understanding of biological systems and processes; Understanding of general principles of physics and basic chemistry for the molecular understanding of biological systems and processes. Understanding of the safety standards for the various types of laboratory. Scientific communication in English. |
| Teaching methods | Lectures, written and oral review. Virtual and practical exercises. |
| Other information | Intermediate test |
| Learning verification modality | Written and oral exam. |
| Extended program | THEORETICAL PART: 1. Sampling and storing techniques 2. Media 3. Sterilization techniques 4. Isolation techniques 5. Microbial micro- and macro-morphologies 6. Selection and enrichment 7. Microbial counting techniques 8. Growth curves modeling PRACTICALS: PART I: LABORATORY 1. Microbial counts 2. Growth curve 3. Basic techniques for in vitro animal cells cultures 4. Cell counts from in vitro animal cell cultures 5. Microscopy of tissues and cells from cells cultures PART II: COMPUTER CLASSROOM Computerized Analysis Tools: 1. Template in MS EXCEL 2. Dynamic Template 3. Elements of macro programming in VBA 4. Template for the microbial counting 5. Template for the growth curve 6. Excel Macro |
Canale B
| CFU | 6 |
|---|---|
| Lecturer | Laura Corte |
| Lecturers |
|
| Hours |
|
| Language of instruction | Italian |
| Contents | THEORETICAL PART: Microbiological Techniques PRACTICALS: PART I Laboratory experience PART II Computerized Analysis Tools |
| Reference texts | Biologia Dei Microrganismi - Gianni Dehò e Enrica Galli – Casa Editrice Ambrosiana |
| Educational objectives | Understanding the structural and functional properties of biological macromolecules, nutrient metabolism and metabolic integrations, regulatory mechanisms operating in biological systems; understanding the structure and biological properties of fungi and bacteria; of the biological structure and properties of cells and animal tissues and methodologies of the molecular analyses. Use and understanding of the animal and microbial models. |
| Prerequisites | Knowledge of mathematical, statistical and bioinformatic methods for quantitative analysis and understanding of biological systems and processes; Understanding of general principles of physics and basic chemistry for the molecular understanding of biological systems and processes. Understanding of the safety standards for the various types of laboratory. Scientific communication in English. |
| Teaching methods | Lectures, written and oral review. Virtual and practical exercises. |
| Other information | Intermediate test |
| Learning verification modality | Written and oral exam. |
| Extended program | THEORETICAL PART: 1. Sampling and storing techniques 2. Media 3. Sterilization techniques 4. Isolation techniques 5. Microbial micro- and macro-morphologies 6. Selection and enrichment 7. Microbial counting techniques 8. Growth curves modeling 9. Bioassay PRACTICALS: PART I: LABORATORY 1. Microbial counts 2. Growth curve 3. Basic techniques for in vitro animal cells cultures 4. Cell counts from in vitro animal cell cultures 5. Microscopy of tissues and cells from cells cultures PART II: COMPUTER CLASSROOM Computerized Analysis Tools: 1. Template in MS EXCEL 2. Dynamic Template 3. Elements of macro programming in VBA 4. Template for the microbial counting 5. Template for the growth curve 6. Excel Macro |