Study-unit BIOSENSORS AND BIOMATERIALS
| Course name | Biotechnology |
|---|---|
| Study-unit Code | A003034 |
| Curriculum | Comune a tutti i curricula |
| Lecturer | Benedetta Carlotti |
| Lecturers |
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| Hours |
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| CFU | 6 |
| Course Regulation | Coorte 2022 |
| Supplied | 2024/25 |
| Learning activities | Affine/integrativa |
| Area | Attività formative affini o integrative |
| Sector | CHIM/02 |
| Type of study-unit | Obbligatorio (Required) |
| Type of learning activities | Attività formativa monodisciplinare |
| Language of instruction | English |
| Contents | Introduction to fluorescence. Use of fluorescent sensors to probe pH, ions, cellular polarity and viscosity, oxygen reactive species, membrane potentials. Use of fluorescent sensors for the specific investigation of different biomaterials. Photodynamic therapy of tumors. |
| Reference texts | David M. Jameson, INTRODUCTION TO FLUORESCENCE, 2014, CRC Press. Iain D. Johnson, MOLECULAR PROBES HANDBOOK-A GUIDE TO FLUORESCENT PROBES AND LABELING TECHNOLOGIES, 2010, InVitrogen. |
| Educational objectives | The student will become familiar with the most important concepts about the choice and use of fluorescent sensors for the investigation of biomaterials. |
| Prerequisites | In order to understand the topics covered in the course, basic knowledge of Molecular Spectroscopy and Chemical Kinetics are required. These issues will be addressed in some lessons that will be held at the beginning of the course. |
| Teaching methods | Lectures with slide projections. Some lessons will be dedicated to the deepening of the addressed issues, some others to the visit of laboratories of our department. |
| Other information | Attending the lectures is not compulsory but strongly recommended. Tutoring activity is planned during the course and for the students who request it to assist them in the preparation of the exam. |
| Learning verification modality | The exam consists in an oral test. The oral examination is a discussion lasting about 30 minutes aimed at ascertaining the level of knowledge and understanding reached by the student on the topics listed in the program. The oral exam will also serve to verify the communication skills of the student and ability in autonomous organization of the exposition. |
| Extended program | Introduction to fluorescence. Light absorption. Instrumentation for fluorescence measurements. Time resolved fluorescence. Anisotropy. FRET. Fluorescence microscopy. Fluorescent sensors to probe pH, ions, cellular polarity and viscosity, oxygen reactive species, membrane potentials. Conjugation of fluorescent sensors to biomolecules: amino reactive probes, thiol reactive probes. Probes for different biomolecules and cellular compartments. Fluorescent proteins. Photodynamic therapy of tumors. |