Study-unit BIOCHEMICAL METHODS
| Course name | Biology |
|---|---|
| Study-unit Code | GP004082 |
| Curriculum | Biomolecolare |
| Lecturer | Serena Porcellati |
| Lecturers |
|
| Hours |
|
| CFU | 6 |
| Course Regulation | Coorte 2022 |
| Supplied | 2022/23 |
| Supplied other course regulation | |
| Learning activities | Affine/integrativa |
| Area | Attività formative affini o integrative |
| Sector | BIO/10 |
| Type of study-unit | Opzionale (Optional) |
| Type of learning activities | Attività formativa monodisciplinare |
| Language of instruction | Italian |
| Contents | Chromatographic techniques. Enzyme assays |
| Reference texts | Keith Wilson and John Walker: Biochimica e biologia molecolare:Principi e tecniche¿ EDs. 2019 Raffaello Cortina Editore. ¿ M.Maccarrone: Metodologie Biochimiche e Biomolecolari, Zanichelli Editore Bonaccorsi, Contestabile, Di Salvo -Metodologie biochimiche-Casa Editrice Ambrosiana. Distrib. Zanichell |
| Educational objectives | Knowledge about the chromatographic methods for the study of the main biomolecules. |
| Prerequisites | Basic knowledge in the field of organic chemistry and biochemistry |
| Teaching methods | Lectures with the help of slides |
| Other information | Optional |
| Learning verification modality | Interview on course topics , within the exam with the other teachers. |
| Extended program | Lab's instruments. Wow to prepare solutions. Protein extraction and cellular lysis by tissue's omogeneization. Methods and techniques for protein purification: centrifugation, fractional precipitation of proteins dialysis. Chromatographic techniques: introduction. Molecular exclusion chromatography. Ion exchange chromatography. Affinity chromatography. Partition chromatography: thin-layer chromatography. Adsorption chromatography. High-pressure liquid chromatography. Enzyme kinetics: Measure of enzyme catalitic activity. Specific activity, Km and Vmax values. Enzymatic inhibition: competitive, uncompetitive. |