Study-unit GENETICS AND GENOMICS
| Course name | Biology |
|---|---|
| Study-unit Code | A001058 |
| Curriculum | Bionutrizionistico |
| Lecturer | Hovirag Lancioni |
| Lecturers |
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| Hours |
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| CFU | 6 |
| Course Regulation | Coorte 2022 |
| Supplied | 2022/23 |
| Supplied other course regulation | |
| Learning activities | Caratterizzante |
| Area | Discipline del settore biomolecolare |
| Sector | BIO/18 |
| Type of study-unit | Obbligatorio (Required) |
| Type of learning activities | Attività formativa monodisciplinare |
| Language of instruction | Italian |
| Contents | Advanced molecular methods for the study of prokaryotic and eukaryotic genomes. Study of genomic complexity. |
| Reference texts | Brown TA – Genomes 4. Edises and other didactic material provided by the teacher |
| Educational objectives | The course aims to broaden the knowledge of Genetics by making students acquire notions and skills of Genetics and Genomics that can be spent in different areas from genomics to evolution, biodiversity, medical genetics, etc. At the end of the course students will have to know how to apply a scientific methodology for understanding the structure and complexity of prokaryotic and eukaryotic genomes, as well as knowing how to participate in group discussion on a research topic. |
| Prerequisites | Genetics |
| Teaching methods | Frontal lessons, laboratory exercises and didactic seminars. |
| Other information | |
| Learning verification modality | Verification will take place through oral examination and through verification of laboratory activities. Critical reading of scientific papers |
| Extended program | Introduction to genomics: definition and traditional disciplines of genetics integrated into genomics. Structural and genetic organization of prokaryotic genomes and eukaryotic organelles. Structural and genetic organization of eukaryotic genomes. The human genome. Development of the Human Genome (HGP) project. Historical notes, cultural and organizational aspects. The main scientific strategies and approaches used for the realization of the HGP and its purposes. Organization of a large-scale sequencing project. Genetic and molecular techniques that allow the study, molecular characterization and annotation of entire genomes. Massive and ultra-massive sequencing techniques and applications used for the study of genomes, the concepts of assembly and genomic annotation, and the methods of investigation of genomic databases to interpret and undertake genomic studies. Second generation sequencing methods. Third generation sequencing methods. The role of bioinformatics. Experimental approaches on a genomic scale that can be used to identify genes and genetic pathways. Methods for studying molecular variability. Metabarcoding and metagenomics. Screening of genomic-scale mutations as a strategy for functional genetic dissection of complex biological processes. Analysis of genomic sequences. Looking for an Open Reading Frame (ORF). Assigning a function to a gene: Computer-based and experimental approaches. Forward and reverse genetics. Evolutionary models and molecular phylogeny. Methods for the creation of phylogenetic trees: based on the calculation of genetic distances (e.g. UPGMA) or on the analysis of individual sites (e.g. Maximum Parsimony). The molecular clock: from molecular divergence to time estimation. Examples of phylogeographic analysis applied to the study of the origin and evolution of modern human. Genetics of human taste perception |