Study-unit BIOCHEMICAL METHODS
Course name | Biology |
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Study-unit Code | GP004082 |
Curriculum | Bionutrizionistico |
Lecturer | Serena Porcellati |
Lecturers |
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Hours |
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CFU | 6 |
Course Regulation | Coorte 2022 |
Supplied | 2022/23 |
Supplied other course regulation | |
Learning activities | Affine/integrativa |
Area | Attività formative affini o integrative |
Sector | BIO/10 |
Type of study-unit | Opzionale (Optional) |
Type of learning activities | Attività formativa monodisciplinare |
Language of instruction | Italian |
Contents | Chromatographic techniques. Enzyme assays |
Reference texts | Keith Wilson and John Walker: Biochimica e biologia molecolare:Principi e tecniche¿ EDs. 2019 Raffaello Cortina Editore. ¿ M.Maccarrone: Metodologie Biochimiche e Biomolecolari, Zanichelli Editore Bonaccorsi, Contestabile, Di Salvo -Metodologie biochimiche-Casa Editrice Ambrosiana. Distrib. Zanichell |
Educational objectives | Knowledge about the chromatographic methods for the study of the main biomolecules. |
Prerequisites | Basic knowledge in the field of organic chemistry and biochemistry |
Teaching methods | Lectures with the help of slides |
Other information | Optional |
Learning verification modality | Interview on course topics , within the exam with the other teachers. |
Extended program | Lab's instruments. Wow to prepare solutions. Protein extraction and cellular lysis by tissue's omogeneization. Methods and techniques for protein purification: centrifugation, fractional precipitation of proteins dialysis. Chromatographic techniques: introduction. Molecular exclusion chromatography. Ion exchange chromatography. Affinity chromatography. Partition chromatography: thin-layer chromatography. Adsorption chromatography. High-pressure liquid chromatography. Enzyme kinetics: Measure of enzyme catalitic activity. Specific activity, Km and Vmax values. Enzymatic inhibition: competitive, uncompetitive. |