Study-unit CHEMICAL AND FERMENTATIVE METHODOLOGIES FOR PRODUCTION OF BIODRUGS
| Course name | Pharmaceutical biotechnologies |
|---|---|
| Study-unit Code | A001037 |
| Curriculum | Comune a tutti i curricula |
| Lecturer | Giuseppe Manfroni |
| Lecturers |
|
| Hours |
|
| CFU | 6 |
| Course Regulation | Coorte 2023 |
| Supplied | 2023/24 |
| Supplied other course regulation | |
| Learning activities | Caratterizzante |
| Area | Discipline farmaceutiche |
| Sector | CHIM/08 |
| Type of study-unit | Obbligatorio (Required) |
| Type of learning activities | Attività formativa monodisciplinare |
| Language of instruction | Italian |
| Contents | Production methods, design and chemical modifications of some biotechnological drugs: siRNA, aptamers, ribozymes. Biotransformations. Penicillins as traditional biotechnological drugs. Industrial processes for the production of biomass and biotechnological products. |
| Reference texts | Printed slides and Pharmaceutical Biothecnology-fundamentals and applications-Third edition-informa healthcare. Edited by Crommelin, Sindelar, Meibohm |
| Educational objectives | To understand the principles of Pharmaceutical Biotechnology with particular regard to the chemical modifications of biotech products. To know the main techniques to analyze, to purify and to manipulate biomasses. |
| Prerequisites | In order to understand the topics covered during the course and achieve the learning objectives, it is necessary to have acquired both chemical and biological basic knowledge to be used as starting platform. In particular, it is essential to have a solid background of organic chemistry and biochemistry. |
| Teaching methods | Frontal lessons by slides projection and practical laboratory experiences followed by written reports. |
| Other information | Please visit the web site of the department to know additional details |
| Learning verification modality | The exam consists of an oral evaluation of about 25 min. The oral test is designed to assess the level of knowledge attained by the student on the theoretical contents covered by the program, his/her logical reasoning, the ability to communicate appropriately and organize coherently the subject matter of the test. In the final evaluation, the written reports of laboratory work will be also considered. The score range is between 18 (lowest) to 30 (highest) with the possibility of honors. |
| Extended program | Lessons (4 ECTS): Antibiotics from natural sources: activity, mechanisms of action industrial fermentative productions. Chemical and productive aspects in biomass processing; chemical reactions through the use of microorganisms and enzymes to obtain biotechnological products. Theoretical aspects of chromatography and polarimetry. Chemical-pharmaceutical aspects of siRNAs, aptamers and ribozymes: chemical modifications, structure-activity relationships and stability. Design of new therapeutic nucleic acids. Examples of RNA biotech drugs: patisiran, givosiran, inclisiran, lumasiran, pegaptanib, angiozyme. After learning the main information regarding the instrumentation, materials and glassware used in a biotechnological laboratory, the student will be able to take part in the laboratory experiences concerning: - F.U. identification reactions, polarimetric analyses, and TLC of penicillin, amino acids and sugars and separation of mixtures; - enantioselective reduction reaction of ethyl acetoacetate through the use of Saccharomyces cerevisiae. |
| Obiettivi Agenda 2030 per lo sviluppo sostenibile | None |