Study-unit BIODRUGS IN CELLULAR AND ANIMAL ENGINEERING
| Course name | Pharmaceutical biotechnologies |
|---|---|
| Study-unit Code | GP003559 |
| Curriculum | Comune a tutti i curricula |
| Lecturer | Francesca Fallarino |
| Lecturers |
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| Hours |
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| CFU | 6 |
| Course Regulation | Coorte 2023 |
| Supplied | 2023/24 |
| Supplied other course regulation | |
| Learning activities | Caratterizzante |
| Area | Discipline farmaceutiche |
| Sector | BIO/14 |
| Type of study-unit | Obbligatorio (Required) |
| Type of learning activities | Attività formativa monodisciplinare |
| Language of instruction | Italian |
| Contents | Classes consist of lectures and practical sections. The students will develop skills in the design, production and screening of a monoclonal antibodies, transgenic and knockout animals. |
| Reference texts | Slides from classes and book: Compendio di biotecnologie Farmaceutiche, Calabrò, Farmaci biotecnologici Casa editrice ambrosiana |
| Educational objectives | The main aim of this teaching course is to provide students with the bases needed for producing a biopharmaceutical as a molecule identical to an endogenous one or an artificial one. The main knowledge acquired will be: • Basic methods for obtaining monoclonal antibodies: • The use of antibodies and proteins as therapeutic agents • Main methods for obtaining engineered cells and animals. The main skills will be: • Produce an hybridoma • The characterization of knockout and transgenic animals. Chimeric receptors and proteins. |
| Prerequisites | In order to understand the content covered and achieve the goals of this course it will be necessary to have the knowledge of the basic techniques of molecular biology and of the principles of general pharmacology. These are the notions that the students should have acquired in his/her school curriculum. The knowledge of these topics is an important prerequisite for the students planning to follow this course with profit. |
| Teaching methods | Theoretical lessons and practical laboratory training. |
| Other information | Following lessons is optional but strongly advised. |
| Learning verification modality | The exam consists an oral exam. This test consists in a discussion lasting about twenty minutes aimed at ascertaining the level of knowledge and understanding achieved by students on the theoretical and practical content of the program (antibodies and proteins as biopharmaceuticals animal engineering for the production of biologically active molecules ). The oral exam will also allow to check the communication skills of the student and his/her autonomy in the organization and exposure of theoretical topics. |
| Extended program | Pharmacogenomic: impact on drug discovery, validation, and response. Molecular basis of drug design, Monoclonal antibodies as biodrugs Monoclonal antibody in immunephenotyping by fluorescence activated cell sorting (FACS) Stem cells and their potential applications in biotecnology. In vivo pharmacology: fully understanding the role of a gene in health and disease. Transgenic animals: methods to obtain transgenics use of trangenic animals in the production of biodrugs Knockout animals: classical and novel methods to obtain knockout animals such as CRISPR knockout animals as tools for drug discovery and target validation. Practical activities on the production of monoclonal antibodies and on knockout mice characterization and target validation. |
| Obiettivi Agenda 2030 per lo sviluppo sostenibile | Study of partnerships and collaborations: In the course, it is possible to explore the importance of partnerships and collaborations among various stakeholders in the field of biopharmaceuticals. This may include case studies of successful interactions among pharmaceutical companies, academic institutions, regulatory bodies, and international organizations that have led to significant outcomes in achieving sustainable development goals. |